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Little is known about his early life but we do know that His mother, Margaretha came from a well-to-do brewer's family, and married Jacbon Jansz Molijn, a painter, after Philips' death. Antony had four older sisters, Margriete, Geertruyt, Neeltge, and Catharina. He married Barbara de Mey in July 1654, with whom he would have one surviving daughter, Maria (four other children died in infancy). Barbara died in 1666, and in 1671 Leeuwenhoek married Cornelia Swalmius, with whom he had no surviving children.