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The transmission electron microscope works similarly to the light microscope however the light rays are replaced by a beam of electrons. These can be focused using magnets in the magnetic/ objective aperture lens. The projector lens then focuses the electrons onto the fluorescent screen and electrons which were scattered by the specimen leave an image.

Resolution: The resolution of a TEM is much higher then that of light microscope. (0.2 nm) This is because the wave length of the electron beam is much shorter.

TEM microscopes

Pros:
-higher resolution so you are able to see finer detail more clearly.
-higher magnification
-higher control of image formation as electrons can be focused using electromagnets due to their charge

Cons:
-cannot observe living specimens
-images are not formed in colour but in grey scale although false colour images are produced
- need to stain specimens and they need to be thin therefore specimens have to be altered a lot sometimes

Uses and effectiveness: The TEM microscopes are great for seeing things in finer details and observing small features. However they are expensive and very expensive so more useful in intricate studies

Suitable for:
- detailed lab work for example research on cell organelles.

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Microscopes

By Jenny