Creating a library

1. Extract and purify DNA from a cell of the organism that contains the gene of interest.

2. Use a restriction enzyme to cut the DNA into manageable sizes. Use the same enzyme to cut the vector you are going to use.

3. Use DNA Ligase to join the DNA fragments that contain the gene of interest with the cleaved vectors.

4. Insert the recombinant plasmid into the bacteria through transformation or electroporation. Amplify the bacterial through natural growth in agarose or through PCR.

5. Store the bacteria in a vial. Keep the vial preserved in cryopreservator, until the bacteria is needed for research.